Is nerve bundle hypertrophy a reliable criteria for diagnosing Hirschsprung disease? A case control study using Calretinin as an adjunct tool for confirming Hirschsprung disease.
نویسندگان
چکیده
Objective: To determine the frequency of nerve bundle hypertrophy in aganglionic segments relation to site biopsy, along with assessment value Calretinin immunostaining diagnosis suspected cases Hirschsprung disease. Study Design: Cross Sectional study. Setting: Department Pathology, Pakistan Institute Medical Sciences (PIMS) Islamabad. Period: September 2018 March 2019. Material & Methods: After routine tissue processing colonic biopsies were examined for presence ganglion cells and hypertrophic or absence. Diagnosis disease was based on absence submucosal myenteric plexus, nerves (more than 4 >30 µm thick/×200 field more 2 >40 field) also noted all (aganglionic segments). applied controls findings recorded as positive negative staining. Statistical Analysis: Data analyzed using SPSS version 23. Qualitative data calculated frequencies percentages. Pearson Chi square test used establish association biopsy. Results: Total 60; 30 each from ganglionic segments. sensitivity our study 90%, specificity 83.3%. In aganglionosis present 13(21.7%) they absent 17 (28.3%). No significant (p value= 0.447) seen Conclusion: immunohistochemistry can be a reliable ancillary technique HD. Aganglionosis may not always associated which alone should criteria HD but instead adjunct methods like must utilized confirm cells. There is no
منابع مشابه
Evaluation of calretinin immunohistochemistry as an additional tool in confirming the diagnosis of Hirschsprung disease.
Hirschsprung disease (HD) is a congenital malformation defined as the absence of myenteric and submucosal ganglion cells (GCs) in the distal rectum and variable length of the contiguous bowel. The aim of this study was to assess the utility of calretinin immunochemistry in comparison with that of standard histology complemented with acetylcholinesterase (AChE) histochemistry routinely employed ...
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Background: Hirschsprung disease is a complex genetic disorder of the enteric nervous system (ENS), often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. The definitive diagnosis of Hirschsprung disease relies on histologic and/or histochemical staining of sections fr...
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ژورنال
عنوان ژورنال: The professional medical journal
سال: 2022
ISSN: ['1024-8919', '2071-7733']
DOI: https://doi.org/10.29309/tpmj/2022.29.09.7027